顯微鏡細(xì)胞介導(dǎo)細(xì)胞毒作用檢測法6：
用含10%小牛血清和抗生素的RPMI-1640培養(yǎng)液將K562細(xì)胞配成1&&time105/ml濃度。用同樣培養(yǎng)液將MC配成1&&time106/ml濃度。2.取96孔圓底細(xì)胞培養(yǎng)板，每孔加0.1ml K562細(xì)胞懸液;每孔加適量MC...
熒光法檢測CM熒光顯微鏡熒光法檢測CMC1.
顯微鏡細(xì)胞介導(dǎo)細(xì)胞毒作用檢測法6：熒光法檢測CMC
1.用含10%小牛和的RPMI-1640培養(yǎng)液將K562細(xì)胞配成1&time105/ml濃度。用同樣培養(yǎng)液將MC配成1&time106/ml濃度。
2.取96孔圓底，每孔加0.1ml K562細(xì)胞懸液;每孔加適量MC使效靶比為1：1～5：1，每種處理3個復(fù)孔;3個對照孔只加效應(yīng)細(xì)胞;8個對照孔只加靶細(xì)胞;4個對照孔只加細(xì)胞培養(yǎng)液。每孔加20l Alamar Blue;每孔總量為0.2ml。
3.在37℃ 5% CO2的飽和水汽二氧化碳培養(yǎng)箱中培養(yǎng)24小時。
4.直接在熒光檢測儀上檢測熒光強(qiáng)度，激發(fā)波長530nm，發(fā)射波長590nm。各孔熒光強(qiáng)度值應(yīng)減去培養(yǎng)液對照熒光強(qiáng)度的平均值，各復(fù)孔熒光強(qiáng)度的平均值記為AF。按下式計算特異性殺傷百分比：
BX53TR Research Microscope RMB238000.00
顯微鏡鏡體
BX53TRF Micrscope frame for transmitted and reflected microscopy with built-in filter holders for four filters(LBD, ND6, ND25, an open position for an optional filter of 45mm dia.), including hexagonal driver AB8804, immersion oil 8cc and shield sticker AD4908(2pcs.)Corresponded to WEEE and RoHS regulations 1
中性灰度濾色片
U-25ND25 Neutral density filter for incident light 1
U-25ND6 Neutral density filter for incident light 1
三目鏡筒
U-TR30-2 Trinocular tube 1
六孔物鏡轉(zhuǎn)盤
U-D6RE Sextuple revolving nosepiece with a slot for analyzer or DICslider 1
機(jī)械式載物臺
U-SVRB-4 Ceramic surface mechanical stage with right-hand low drivecontrol(long type) 1
夾片器
U-HLDT-4 Specimen holder for BX stage, left hand, for two specimens, thick type 1
100W鹵素?zé)魺羰?br />
U-LH100-3 Lamp house for 100W halogen with connecting cable Corresponded to RoHS, WEEE regulations 1
100W汞燈燈室
U-LH100HG Lamp house for 100W mercury burner with collector lens and connecting cable 1
汞燈電源
U-RFL-T Power supply unit for Mercury lamp meeting the RoHS regulation 1
12V100W燈泡
JC12V100WHAL-L Halogen bulb(Philips No. 7724) 2
電源線
UYCP Power cord 2
熒光激發(fā)塊
U-MWB2 Mirror Unit for fluorescence microscopy, wide band Blue excitation, exciter filter BP450-480, dichroic beamsplitter DM500, barrier filter BA515 1
U-MWG2 Mirror Unit for fluorescence microscopy, wide band Green excitation, exciter filter BP510-550, dichroic beamsplitter DM570, barrier filter BA590 1
熒光反射器
BX-URA2 Universal reflected light illuminator with field stop and aperture stop, including 6-position mirror unit cassette, UV protective shield AD5417, shield plate AB1550, Allen wrench AD6162 and indication plate AD5419 1
防塵罩
COVER-018 Dust cover Type 018 for BX2(L 510mm x W 320mm x H 530 mm) 1
汞燈燈泡
USH103OL 100W mercury burner for U-LH100HGAPO, U-LH100HG 2
目鏡
WHN10X Widefield eyepiece 10X 2
無熒光鏡油
IMMOIL-F30CC Immersion oil 30cc. Low level auto-fluorescence. 1
相差附件
BX2-PHD-P11 Phase Contrast/Darkfield Attachment 1
相差聚光鏡
U-PCD2 Phase contrast dark field condenser NA1.1 1
平場消色差相差物鏡
PLN10XPH Plan achromat phase objective 10X/0.25, WD 10.6 1
PLN20XPH Plan achromat phase objective 20X/0.4, WD 1.2(spring) 1
PLN40XPH Plan achromat phase objective 40X/0.65, WD 0.6(spring) 1
PLN100XOPH Plan achromat phase objective 100X/1.25, WD 0.15(spring, oil) 1
對中望遠(yuǎn)鏡
U-CT30 Centering telescope 1
濾色片
43IF550-W45 Interference green contrast filter 45mm dia. 1
簡易偏光裝置
BX2-POL Simple Polarizing Attachment 1
起偏器
U-POT Polarizer for transmitted light 1
檢偏器
U-ANT Analyzer for transmitted light 1
DP72 1200萬像素 沒必要買DP73
MP5.0-RTV-R-CLR-10-C MicroPublisher 5.0 RTV, 5 mega-pixel CCD Color-Bayer Mosaic, 10-bit, cooled, Real Time Viewing 1 RMB140000
C型視頻接口
U-TV0.5XC-3 C-mount video attachment with 0.5X lens 1 RMB8000.00
IPP-60 軟件 解密版 5000元
